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313257

Invasive Versus Noninvasive Methods for Diagnosis of Helicobacter Pylori infection

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Helicobacter pylori (H. pylori) is one of the most widespread infectious organisms in the world that is related to many gastrointestinal diseases. There are numerous techniques available at the present time for identifying infections caused by H. pylori, each has advantages and disadvantages. Objectives: To compare invasive versus noninvasive techniques used for diagnosis of H. pylori infections. Methodology: The study was performed on 100 patients complaining of gastrointestinal problems undergoing GIT endoscopy admitted to Gastroenterology Department, Benha University Hospital. Two samples of gastric biopsy were taken from each patient ; one  used for bacteriological culture and one used for Polymerase chain reaction (PCR), 100 stool samples were taken for stool antigen test (SAT), and 100 serum samples for Enzyme-Linked Immunosorbent Assay ( ELISA). Results: Out of 100 biopsy samples, 71 (71%) had been positive for PCR, 70 (70 %) had been positive for culture. Out of 100 stool sample, 64 (64%) had been positive for SAT. Out of 100 serum samples, 68 (68%) had been positive for ELISA. In comparison with PCR, the culture showed 98.6 % sensitivity, 89.7 % specificity, and total accuracy of 96% then SAT with 88% accuracy and ELISA showed 86% accuracy. Conclusion: Considering PCR as the gold standard method for H. pylori diagnosis, the culture showed the highest overall performance followed by SAT and ELISA.

DOI

10.21608/ejmm.2023.313257

Keywords

Helicobacter pylori, PCR, culture, SAT, ELISA

Authors

First Name

Rasha

Last Name

El sayed

MiddleName

A.

Affiliation

Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

rasha_micro@yahoo.com

City

benha

Orcid

-

First Name

Mysa

Last Name

Mostafa

MiddleName

S.

Affiliation

Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

mysasaid@yahoo.com

City

Benha

Orcid

-

First Name

Ahmed

Last Name

Mahedy

MiddleName

W.

Affiliation

Internal Medicine Department, Faculty of Medicine, Benha University, Benha, Egypt

Email

wagehhmd@yahoo.com

City

benha

Orcid

-

First Name

Kawthar

Last Name

Mohamed

MiddleName

I.

Affiliation

Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University, Egypt

Email

kawthar_75@outlook.sa

City

-

Orcid

-

Volume

32

Article Issue

4

Related Issue

42768

Issue Date

2023-10-01

Receive Date

2023-08-16

Publish Date

2023-10-01

Page Start

17

Page End

22

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 4_Pages 17-22.pdf

PDF . 369.8kB

Link

https://ejmm.journals.ekb.eg/article_313257.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=313257

Order

313,257

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Invasive Versus Noninvasive Methods for Diagnosis of Helicobacter Pylori infection

Details

Type

Article

Created At

28 Dec 2024