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311250

Insights into Biofilm-based Multidrug Resistance in Acinetobacter Baumannii Isolated from Patients Hospitalized in Benha University Intensive Care Unit

Article

Last updated: 28 Dec 2024

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Abstract

Background: Acinetobacter baumannii (A.baumannii) is an opportunistic pathogen that causes serious types of infections in human. It shows a wide range of antibiotic resistance. Objective: to investigate biofilm formation and its relation to biofilm-related genes in multidrug-resistant (MDR) A.baumannii isolated from patients hospitalized in Benha University Intensive Care Unit (ICU). Methodology: This study was conducted on (90) clinical samples obtained from immunocompromised patients with various symptomatic clinical infections, at least 48 hours after admission to Benha University ICU. A. baumannii isolates were identified, their antibiotic susceptibility pattern was detected using VITEK® 2 system. Biofilm-forming capacity of A. baumannii isolates was determined by tube method. Biofilm-related genes (csuE , omp A, bap , bla PER-1, and aba I) were detected using polymerase chain reaction(PCR). Results: Out of 60 A.baumannii isolates, 93.3% were MDR, 76.7% were extensive drug resistant (XDR), and 75% were biofilm formers. A significant relationship was revealed between the multidrug resistance and biofilm formation. Detected ratio of csuE, omp A, bap, bla PER-1,, and aba I genes were 96.7%, 48.3% , 68.3% , 40% and 88.3% respectively. (csu E, bap, aba I, and bla PER-1) genes were founded to have a statistically significant relation to biofilm formation. While (omp A) gene was insignificantly related to biofilm formation. Conclusion: Biofilm formation in A. baumannii is significantly related to multidrug resistance, with high frequency of biofilm -related genes.

DOI

10.21608/ejmm.2023.311250

Keywords

Virulence, PCR, Antimicrobial

Authors

First Name

Mysa

Last Name

Mostafa

MiddleName

S.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine Benha University, Egypt

Email

mysasaid@yahoo.com

City

Benha

Orcid

-

First Name

Ahmed

Last Name

Mahedy

MiddleName

W.

Affiliation

Department of Internal Medicine, Faculty of Medicine, Benha University, Egypt

Email

-

City

Benha

Orcid

-

First Name

Asmaa

Last Name

Ebaed

MiddleName

B.

Affiliation

Department of Critical Care Medicine, Faculty of Medicine, Benha University, Egypt

Email

asmaa.bahy8819@gmail.com

City

Benha

Orcid

-

First Name

Samah

Last Name

Ebaed

MiddleName

Bahy Mohammed

Affiliation

Department of Clinical Pharmacology, Faculty of Medicine, Benha University, Egypt

Email

samahbahi2005@gmail.com

City

Benha

Orcid

-

First Name

Taghrid

Last Name

Kharboush

MiddleName

G.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine Benha University, Egypt

Email

t.g.kharboush@gmail.com

City

-

Orcid

-

Volume

32

Article Issue

4

Related Issue

42768

Issue Date

2023-10-01

Receive Date

2023-08-05

Publish Date

2023-10-01

Page Start

1

Page End

8

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 4_Pages 1-8.pdf

PDF . 616.7kB

Link

https://ejmm.journals.ekb.eg/article_311250.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=311250

Order

311,250

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Insights into Biofilm-based Multidrug Resistance in Acinetobacter Baumannii Isolated from Patients Hospitalized in Benha University Intensive Care Unit

Details

Type

Article

Created At

28 Dec 2024