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316281

Influence of Diclofenac and Pheniramine on the Virulence of Different Clinical Isolates of Pseudomonas aeruginosa

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Objectives: This study aimed to examine the effect of commonly used non-antibiotic drugs e.g. diclofenac and pheniramine, Background: Treatment of Pseudomonas aeruginosa infections, on antibiotic resistance and the virulence of this pathogen. The antibiotics: gentamicin, cefepime, ciprofloxacin and meropenem were investigated in this study. Methodology: In this work we selected the following final concentrations: 1,4µg/ml for diclofenac and 0,173µg/ml for pheniramine to be used in combination with antibiotics or alone for investigation of their effects on antibiotic resistance and virulence of 20 isolates of P. aeruginosa. Results: The drugs either increased or decreased antibiotic resistance in only 3 isolates of the 20 isolates which indicated that the investigated drugs did not affect the antibiotic resistance when used in combinations. Interestingly, our study demonstrated that both diclofenac and pheniramine increased the haemolytic activity of the investigated isolates. On the other side, no overall final increasing or decreasing effect could be observed regarding the effect of diclofenac or pheniramine on the proteolytic activity of the investigated isolates. The results were confirmed by Real time PCR  diclofenac showed a significant down- regulation of virulance genes namely; algD, plch and toxA apperently  in case of combination  with ciprofloxacin and to a lower extent when combined with gentamicin. Conclusion: Resistance of P. aeruginosa to Gentamicin and Ciprofloxacin may be successfully affected by combining these antibiotics with Diclofenac.

DOI

10.21608/ejmm.2023.316281

Keywords

P. aeruginosa, resistance, antibiotics, Diclofenac, Virulence

Authors

First Name

Heba

Last Name

AboElfarh

MiddleName

E.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Egypt

Email

heba.aboelfarh.elsaeed@gmail.com

City

Mansoura

Orcid

-

First Name

Mohamed

Last Name

ELSokkary

MiddleName

M

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Egypt

Email

melsokkary@mans.edu.eg

City

Mansoura

Orcid

-

First Name

Elsayed

Last Name

Habib

MiddleName

E.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Egypt

Email

sayedhabib2011@yahoo.com

City

Mansoura

Orcid

-

Volume

32

Article Issue

3

Related Issue

41216

Issue Date

2023-07-01

Receive Date

2023-09-06

Publish Date

2023-07-01

Page Start

133

Page End

142

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 3_Pages 133-142.pdf

PDF . 580.2kB

Link

https://ejmm.journals.ekb.eg/article_316281.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=316281

Order

316,281

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Influence of Diclofenac and Pheniramine on the Virulence of Different Clinical Isolates of Pseudomonas aeruginosa

Details

Type

Article

Created At

28 Dec 2024