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307754

Isolation of Gram-Negative Organisms Causing Nosocomial Catheter Associated Urinary Tract Infection and Detection of Fosfomycin Effect on Multi-Drug Resistant Strains in Sohag Un

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background:  CAUTIs are the commonest HAIs and frequently caused by MDR Gram negative organisms, fosfomycin could be potentially used for this infection. Objectives: To isolate, identify Gram negative organisms causing CAUTIs, their antibiotic resistance pattern and to investigate their sensitivity to fosfomycin at Sohag University Hospitals. Methodology: Cross-sectional study included patients with catheter associated urinary tract infections (CAUTIs) admitted in the different Departments of Sohag University Hospitals from December 2019 to December 2022. The study included 310 urine samples from CAUTIs. Urine samples were subjected to routine culture, complete biochemical identification and antibiotic sensitivity testing were done by the BD Phoenix™ automated identification and susceptibility testing system. ESBL producing organisms were detected by double-disk synergy test (DDST), Carbapenemase production by the modified Hodge test (MHT) and MBL production by EDTA combined disk synergy test (CDST). Fosfomycin sensitivity was detected by the disc diffusion method and minimum inhibitory concentration (MIC) by E-test. Conventional PCR was used to detect FosA, fosA2, fosA3, fosC2 genes. Results: The study group included (180) patients from them Gram negative bacilli were isolated. Their mean age was 52.8±11.7 years. Males were 99 (55%) of all studied patients. Most of them were from ICU. The most common isolated organism was E. coli 75 (41.7%), followed by Klebsiella pneumoniae 39 (21.7%), and Enterobacter cloacae 24 (13.3), 156 (86.6%) were MDR, 67 (43.3%) were ESBL producers by DDST, 49 (27%) Carbapenemase producers by MHT and 40 (22%) were MBL producers by EDTA CDST. One hundred and fifteen strains (66%) were sensitive to fosfomycin by E- test and FosA, A2, A3, C2 genes were detected in 47 (72.3%) of fosfomycin resistant strains.  Conclusion: The study revealed that 86% of CAUTI were caused by MDR Gram negative organisms and 59% of them were susceptible to fosfomycin.

DOI

10.21608/ejmm.2023.307754

Keywords

CAUTI, MDR GN bacilli, ESBL, MBL, fosA, fosA2, fosA3, fosC2

Authors

First Name

Nadia

Last Name

Salman

MiddleName

A. M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Sohag University, Egypt

Email

-

City

Sohag

Orcid

-

First Name

Mona

Last Name

Mohamed

MiddleName

F.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Sohag University, Egypt

Email

aboelsoadsamah@gmail.com

City

Sohag

Orcid

-

First Name

Wesam

Last Name

Abu Elwafa

MiddleName

A.

Affiliation

Department of Anesthesia and Intensive Care, Faculty of Medicine, Sohag University, Egypt

Email

-

City

Sohag

Orcid

-

First Name

Asmaa

Last Name

Goda

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Sohag University, Egypt

Email

asmaa_gouda@med.sohag.edu.eg

City

-

Orcid

0000-0002-2161-7267

Volume

32

Article Issue

3

Related Issue

41216

Issue Date

2023-07-01

Receive Date

2023-07-14

Publish Date

2023-07-01

Page Start

99

Page End

108

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 3_Pages 99-108.pdf

PDF . 557.7kB

Link

https://ejmm.journals.ekb.eg/article_307754.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=307754

Order

307,754

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Isolation of Gram-Negative Organisms Causing Nosocomial Catheter Associated Urinary Tract Infection and Detection of Fosfomycin Effect on Multi-Drug Resistant Strains in Sohag University Hospital

Details

Type

Article

Created At

28 Dec 2024