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304581

Prevalence of E. coli Pathotypes: A Comparative Study between Clinical and Environmental Isolates

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Pathogenicity and virulence

Abstract

Background: The pervasive species of Escherichia coli range from avirulent to extremely pathogenic strains. Pathogenic strains are a serious public health concern globally, causing gastrointestinal infections or disseminate throughout the body, causing  urinary tract infections, and sepsis/meningitis. Among bacterial etiologic agents of gastrointestinal infections, diarrheagenic Escherichia coli (DEC) is the predominant cause of severe diarrhea. Objective: This study aims at determining the prevalence of Escherichia coli pathotypes and serotypes among clinical and environmental isolates. Methodology: A total of 105 presumptive isolates of E. coli were obtained from different clinical (118) and environmental (217) specimens. Confirmed E. coli isolates were subjected to serological identification, as well as determination of pathotypes. Statistical data analysis was performed applying Fisher's exact test. Results: Of the 335 presumptive specimens, 31.3% (105/335) were confirmed as E. coli. Seropathotyping of the confirmed isolates showed their distribution as 49.5% EHEC, 26.7% EPEC, 18.1% ETEC, and 5.7% EIEC. Alarmingly, high rate of EHEC and ETEC were observed among dairy and meat products (50% and 20%, respectively), while a low rate belonged to EIEC pathotype. Concerning E. coli clinical isolates, EHEC followed by EPEC were the most prevalent pathotypes. Regarding serotypes distribution, the most prevalent serotype among environmental isolates was O26: H11, whereas the most common serotype among clinical isolates was O128: H2. Serotypes O26: H11 and O125: H21 were significantly more prevalent among environmental isolates than clinical isolates, while serotypes O126: H21, O55: H7, O119: H6, and O128: H2 were significantly more prevalent among clinical isolates. Conclusion: This research emphasizes the issue of pathogenic pathotypes becoming progressively prevalent in Egypt. We concluded that pathogenic E. coli has been detected not solely in hospitals, but also in food and dairy products rendering them to be possible reservoirs and vehicles for this pathogen.
 

DOI

10.21608/ejmm.2023.304581

Keywords

Escherichia coli, pathotypes, DEC, Serotyping

Authors

First Name

Omnia

Last Name

Bahgat

MiddleName

T.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Email

omniatawfeek6@gmail.com

City

Mansoura

Orcid

-

First Name

Dina

Last Name

Rizk

MiddleName

E.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Email

dena@mans.edu.eg

City

-

Orcid

0000-0003-1609-1878

First Name

Hany

Last Name

Kenawy

MiddleName

I.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Email

hanykenawy@mans.edu.eg

City

Mansoura

Orcid

0000-0002-8548-6649

First Name

Rasha

Last Name

Barwa

MiddleName

-

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Email

rasha@mans.edu.eg

City

Mansoura

Orcid

0000-0003-3130-3576

Volume

32

Article Issue

3

Related Issue

41216

Issue Date

2023-07-01

Receive Date

2023-06-20

Publish Date

2023-07-01

Page Start

59

Page End

69

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 3_Pages 59-69.pdf

PDF . 423.5kB

Link

https://ejmm.journals.ekb.eg/article_304581.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=304581

Order

304,581

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Prevalence of E. coli Pathotypes: A Comparative Study between Clinical and Environmental Isolates

Details

Type

Article

Created At

28 Dec 2024