Beta
ManageSearchChatDiscover
299696

Effect of Lactobacillus acidophilus and Lactobacillus johnsonii on Growth, Phenotypic and Genotypic Expression of Virulence Factors of Candida albicans Causing Vulvovaginal Candi

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Vulvovaginal candidiasis (VVC) causes significant morbidity among women of reproductive age, as well as a considerable financial burden on the health-care system. In the age of antibiotic resistance, using probiotic lactobacilli to treat VVC has become an appealing treatment approach. Objective: The goal of this research was to determine how L. acidophilus LA-5 and L. johnsonii B-2178 affect C. albicans growth, phenotypic and genotypic expression of virulence factors (biofilm and hyphal production) in females with VVC. Methodology: This study was conducted on 30 isolates of C. albicans recovered from 118 patients in the reproductive period, complaining of cheesy curd-like vaginal discharge and/or itching. The effect of L. acidophilus LA-5 and L. johnsonii B-2178 on growth of C. albicans isolates was determined. The effect of L. acidophilus LA-5 and L. johnsonii B-2178 cell-free supernatant (CFS) on phenotypic expression of virulence factors (biofilm and hyphae formation) among C. albicans isolates was determined. By using Quantitative Real-Time Reverse Transcription PCR (qRT-PCR), the effect of CFS of L. acidophilus LA-5 and L. johnsonii B-2178 on the transcription of C. albicans genes involved in the transition from yeast to hyphae and biofilm formation was determined. Results: Both L. acidophilus LA-5 and L. johnsonii B-2178 were effective in inhibiting C. albicans growth. The CFS of L. acidophilus LA-5 and L. johnsonii B-2178 significantly inhibited biofilm formation. The CFS of L. acidophilus LA-5 and L. johnsonii B-2178 reduced hyphae formation, but this reduction was not significant. The CFS of L. acidophilus LA-5 and L. johnsonii B-2178 significantly inhibited expression levels of virulence-related genes. Conclusions: L. acidophilus LA-5 and L. johnsonii B-2178 had growth inhibition effect, anti-biofilm effect, anti-hyphal effect and gene expression level inhibition on C. albicans isolates causing VVC.

DOI

10.21608/ejmm.2023.299696

Keywords

L. acidophilus LA-5, L. johnsonii B-2178, Virulence factors, C. albicans, Vulvovaginal candidiasis

Authors

First Name

ِAliaa

Last Name

Abd El-Aala

MiddleName

A.

Affiliation

Assistant lecturer of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

aliaa_ashraf13@yahoo.com

City

Cairo

Orcid

Aliaa Ashraf Abd El-Aala

First Name

Mohammed

Last Name

Ismail

MiddleName

A.

Affiliation

Professor of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

amrkholy@hotmail.com

City

Cairo

Orcid

-

First Name

Alaa

Last Name

Awad

MiddleName

R.

Affiliation

Professor of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

alaa.mreda@gmail.com

City

Cairo

Orcid

-

First Name

Mohammed

Last Name

El Sharkawy

MiddleName

A.

Affiliation

Professor of Gynecology and Obstetrics, Faculty of Medicine, Cairo University, Cairo, Egyp

Email

kingdoc82@outlook.com

City

Cairo

Orcid

-

Volume

32

Article Issue

3

Related Issue

41216

Issue Date

2023-07-01

Receive Date

2023-05-20

Publish Date

2023-07-01

Page Start

41

Page End

49

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 3_Pages 41-49.pdf

PDF . 507.6kB

Link

https://ejmm.journals.ekb.eg/article_299696.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=299696

Order

299,696

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Effect of Lactobacillus acidophilus and Lactobacillus johnsonii on Growth, Phenotypic and Genotypic Expression of Virulence Factors of Candida albicans Causing Vulvovaginal Candidiasis

Details

Type

Article

Created At

28 Dec 2024