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299226

Detection of Pathogenicity Island-encoding Virulence Genes of Staphylococcus aureus Isolated from Various Clinical Sources

Article

Last updated: 05 Jan 2025

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Tags

Pathogenicity and virulence

Abstract

Background: Staphylococcus aureus is an important pathogen that causes a wide range of diseases in humans and animals. S. aureus pathogenicity islands (SaPIs) have a growing family of mobile genetic elements (MGEs) in Staphylococci. MGEs transferred Horizontally play an important part in the evolution of the pathogenic bacteria (S. aureus). Several SaPIs carry staphylococcal enterotoxin and many toxin genes. Objective: The goal of this study is to screen the pathogenicity islands-encoding virulence genes of S. aureus, determine antimicrobial resistance pattern and evaluate the distribution of virulence genes among these isolates. Methodology: A total of 108 S. aureus clinical isolates were identified and antimicrobial sensitivity pattern for twelve antimicrobial agents from different classes was assessed. In addition, Polymerase chain reaction (PCR) was performed for all isolates to detect virulence genes encoding on SaPI. PCR products were purified by The Thermo Scientific GeneJET Gel Extraction Kit. Nucleotide sequencing analysis by using 3500 Genetic Analyzer Version 6.0, (Applied Biosystems™). Using Sanger method, sequences data were assembled by GeneMapper™ secondary analysis software. The data analysis was done by using CLC Sequence Viewer 8 (clc-sequence-viewer.software.informer.com). Alignment of sequences by BLAST search of DNA Data Bank was performed. Identification of ORFs (Open Reading Frame) by using ORF Finder. Results: Out of the 108 S. aureus clinical isolates, 69 isolates (63.88%) were Methicillin-resistance Staphylococcus aureus (MRSA), 24 isolates (22.22%) were Vancomycin-resistance Staphylococcus aureus (VRSA), and 15 isolates (13.88%) were Methicillin-sensitive Staphylococcus aureus (MSSA). Eight hundred sixty-two nucleotides product aligned with groEL gene that encode Chaperonin GroEL protein, and with grol gene that encode Heat shock protein chaperone GroEL. The sequence 708 bp aligned with int gene encodes Integrase, groEL gene, grol gene and ktrB gene (encodes Potassium uptake protein). The sequence 493 bp aligned with hys gene encodes Hyaluronate lyase enzyme and pLys gene encodes Polysaccharide lyase enzyme. The sequence 366bp and 340bp aligned with metQ gene that encode with permease (MetQ/NIpA transporter protein). Conclusion: Identification of Pathogenicity Island (SaPI) virulence genes can provide useful information for understanding the pathogenicity, drug resistance and horizontal genetic transfer that play a vital part within the evolution of S. aureus.

DOI

10.21608/ejmm.2023.299226

Keywords

S. aureus, S. aureus pathogenicity islands, Open Reading Frame, MRSA, VRSA, MSSA

Authors

First Name

fatema

Last Name

el-naggar

MiddleName

wael

Affiliation

Mansoura University Hospitals, Mansoura University, Mansoura 35516, Egypt

Email

fatema.elnaggar@gmail.com

City

-

Orcid

-

First Name

Soha

Last Name

Elshaer

MiddleName

L.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt

Email

-

City

Mansoura

Orcid

-

First Name

Shaymaa

Last Name

Abdel-Rhman

MiddleName

-

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt

Email

-

City

Mansoura

Orcid

-

First Name

Hany

Last Name

Kenawy

MiddleName

I.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt

Email

hanykenawy@mans.edu.eg

City

Mansoura

Orcid

0000-0002-8548-6649

First Name

Ramadan

Last Name

Hassan

MiddleName

-

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt

Email

rhassan33@gmail.com

City

Mansoura

Orcid

-

Volume

32

Article Issue

3

Related Issue

41216

Issue Date

2023-07-01

Receive Date

2023-05-15

Publish Date

2023-07-01

Page Start

19

Page End

30

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 3_Pages 19-30.pdf

PDF . 766.5kB

Link

https://ejmm.journals.ekb.eg/article_299226.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=299226

Order

299,226

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Detection of Pathogenicity Island-encoding Virulence Genes of Staphylococcus aureus Isolated from Various Clinical Sources

Details

Type

Article

Created At

28 Dec 2024