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298797

Detection of carbapenemase genes and other resistance mechanisms in carbapenem-resistant/cephalosporin-susceptible Pseudomonas aeruginosa

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Antimicrobial agents
Clinical microbiology

Abstract

Background: Carbapenem- resistant P. aeruginosa isolates are increasingly observed. Carbapenem resistance in P. aeruginosa is modulated by acquired carbapenemases in association with non-carbapenemases mechanisms. An uncommon phenotype of carbapenem resistant but cephalosporine susceptible (Carb- R/ Ceph- S) Ps. aeruginosa clinical isolates have been reported. Objective: We aimed to assess mechanisms of carbapenem resistant in this characteristic uncommon phenotype. Methodology: A total of 168 carbapenem resistant clinical isolates of Ps. aeruginosa were recovered form admitted cases in a Tertiary Care Hospital during the period from November 2021 to October 2022. All Carb- R/ Ceph- S Pseudomonas aeruginosa isolates were subjected to the following to detect carbapenems resistant mechanism (s):  Genotypic discovery of carbapemenases production, phenotypic detection both of AmpC overproduction and efflux pumps overproduction. Results: 48 isolates (28.6%) were cephalosporine susceptible (Carb-R/ Ceph-S). Genotypic discovery of carbapenemases encoding genes by multiplex PCR and phenotypic detection of AmpC overproduction and efflux pumps overproduction were done to identify the possible mechanisms of carbapenem resistant in the studied phenotypes. None of 48 Carb- R/ Ceph-S P. aeruginosa isolates were carrying carbapenemases encoding genes, 60.4% (29/48) had efflux pumps overproduction and 4.2% (2/48) had AmpC overproduction. The highest rate of antimicrobial resistance was to Tigecycline and Colistin and the smallest rate of antimicrobial resistance was to Piperacillin/ Tazobactam, and Tobramycin and Amikacin. Conclusions: None of Carb-R/ Ceph-S P. aeruginosa harboring carbapenemases encoding genes, whereas;   efflux pumps overproduction and AmpC overproduction were detected in 60.4% and 4.2% respectively.

DOI

10.21608/ejmm.2023.298797

Keywords

Pseudomonas aeruginosa, Carbapenem resistance, Cephalosporine susceptible. carbapenemase, carbapenem resistant/cephalosporine susceptible Ps. aeruginosa

Authors

First Name

ABDELRAHMAN

Last Name

ELSAWY

MiddleName

-

Affiliation

Medical Microbiology Department, AlAzhar Faculty of medicine, Cairo, Egypt

Email

elsawyeg@gmail.com

City

Makkah

Orcid

-

First Name

Khalid

Last Name

Al-Quthami

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

kalquthami@moh.gov.sa

City

-

Orcid

-

First Name

Ammar

Last Name

Abbas

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

amahabbas@moh.gov.sa

City

-

Orcid

-

First Name

Hind

Last Name

Khan

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

hikhan@moh.gov.sa

City

-

Orcid

-

First Name

Wajanat

Last Name

Hanawi

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

wajanath@moh.gov.sa

City

-

Orcid

-

First Name

Naelah

Last Name

Hanawi

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

naelahh@moh.gov.sa

City

-

Orcid

-

First Name

Shada

Last Name

Almowaled

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabi

Email

salmawaled@moh.gov.sa

City

-

Orcid

-

First Name

Renan

Last Name

Ghazawi

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

rghazzawi@moh.gov.sa

City

-

Orcid

-

First Name

Shirin

Last Name

Kaki

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

skaki@moh.gov.sa

City

-

Orcid

-

First Name

Salwa

Last Name

Andejani

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabi

Email

sandejani@moh.gov.sa

City

-

Orcid

-

First Name

Rakan

Last Name

Felemban

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

rffelemban@moh.gov.sa

City

-

Orcid

-

First Name

Razaz

Last Name

Brashi

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

rbrashi@moh.gov.sa

City

-

Orcid

-

First Name

Eyad

Last Name

Oqap

MiddleName

-

Affiliation

Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia

Email

eeqab@moh.gov.sa

City

-

Orcid

-

First Name

Ashraf

Last Name

Sorour

MiddleName

Elsayed

Affiliation

Medical Microbiology and Immunology Department, Faculty of medicine – Cairo University, Cairo, Egypt

Email

ashraf.sorour@kasralainy.edu.eg

City

-

Orcid

0000-0002-1611-8500

Volume

32

Article Issue

3

Related Issue

41216

Issue Date

2023-07-01

Receive Date

2023-05-13

Publish Date

2023-07-01

Page Start

13

Page End

18

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 3_Pages 13-18.pdf

PDF . 210.8kB

Link

https://ejmm.journals.ekb.eg/article_298797.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=298797

Order

298,797

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Detection of carbapenemase genes and other resistance mechanisms in carbapenem-resistant/cephalosporin-susceptible Pseudomonas aeruginosa

Details

Type

Article

Created At

28 Dec 2024