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298443

Prevalence of Integrons and mcr-mediated resistance among colistin-resistant Enterobacterales in hospitalized patients

Article

Last updated: 28 Dec 2024

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Colistin is a last therapeutic option for carbapenem-resistant Enterobacterales (CRE) infections. Emergence of plasmid-mediated mcr colistin resistance genes poses a potential threat for treatment of these infections. Integrons are known for their central role in antibiotic resistance. Objectives: The aim of the study was to survey the prevalence of mcr-genes and integrons in colistin-resistant Enterobacterales isolates and to assess methods for colistin susceptibility testing. Methodology: Eighty-six colistin-resistant Enterobacterales strains isolated from different clinical samples of hospitalized patients at King Saud Medical City, Riyadh, from February 2020 to December 2021 were included in the study. Antimicrobial susceptibility testing (AST) was done using Phoenix BD, Sensititre broth microdilution test, and the standard broth microdilution method (BMD). Screening for mcr-1 and mcr-2 genes and class I, II and III integrons was done by PCR. Results: Majority of the colistin-resistant isolates (97.6%) were Klebsiella pneumoniae. Categorial agreement (CA) of Sensititre broth microdilution test and Phoenix BD test with the reference BMD method for colistin susceptibility was 95.3% and 89.5%, respectively. Only 3 isolates (3.5%) were found positive for mcr-1 gene. Class I and II integrons were detected in 79. % and 30.2% of the isolates, respectively. Conclusion: Sensititre broth microdilution test displayed a good performance for colistin AST, while Phoenix BD results should be confirmed by another method. Plasmid borne mcr-mediated resistance plays a minor role in colistin resistance among Klebsiella pneumoniae isolates. The alarming high prevalence of antibiotic resistant class I and II integrons in the studied isolates warrants further investigations.

DOI

10.21608/ejmm.2023.298443

Keywords

Enterobacterales, mcr-gene, Integrons, Phoenix BD2, Sensititre

Authors

First Name

Dalia

Last Name

ElFeky

MiddleName

Saad

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

dalia_saadelfeky@kasralainy.edu.eg

City

-

Orcid

-

First Name

Nahla

Last Name

Melake

MiddleName

Ali

Affiliation

2Department of Pathology, College of Medicine, Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia; Department of Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University, Egypt

Email

nahlamelake@yahoo.com

City

-

Orcid

-

First Name

Abdulwahab

Last Name

Binjomah

MiddleName

Zaid

Affiliation

Microbiology Department, Riyadh Regional Laboratory & Blood Bank, Riyadh, Saudi Arabia; College of Medicine, Alfaisal University, Riyadh, Saudi Arabia

Email

abinjomah@moh.gov.sa

City

-

Orcid

-

First Name

Fatimah

Last Name

Al Hulaily

MiddleName

Fuad

Affiliation

Microbiology Department, Riyadh Regional Laboratory & Blood Bank, Riyadh, Saudi Arabia

Email

fal-hulaily@moh.gov.sa

City

-

Orcid

-

First Name

Naif

Last Name

Almutairi

MiddleName

Dakhel

Affiliation

Microbiology Department, Riyadh Regional Laboratory & Blood Bank, Riyadh, Saudi Arabia

Email

naif225@hotmail.com

City

-

Orcid

-

First Name

Khawlah

Last Name

aldilaijan

MiddleName

Essa

Affiliation

Genetic Section, Research Department, ‏Health Sciences Research Center, ‏Princess Nourah bint Abdulrahman University

Email

kealdilaijan@pnu.edu.sa

City

-

Orcid

-

First Name

Lubna

Last Name

Alsaleem

MiddleName

Saleh

Affiliation

Medical Student, College of Medicine, Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia

Email

lsalsaleem63@sm.imamu.edu.sa

City

-

Orcid

-

Volume

32

Article Issue

3

Related Issue

41216

Issue Date

2023-07-01

Receive Date

2023-05-09

Publish Date

2023-07-01

Page Start

1

Page End

11

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_298443.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=298443

Order

1

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Prevalence of Integrons and mcr-mediated resistance among colistin-resistant Enterobacterales in hospitalized patients

Details

Type

Article

Created At

28 Dec 2024