Background: The increase of morbidity of infected patients with multi-drug resistant organisms (MDROs) necessitated use of alternative drugs as tigacycline and colistin. Methodology: A total of 172 isolates were tested for detection of MDR, XDR, and PDR bacteria, testing biofilm-formation by CRA and TCP methods, combined disc test for ESBL production among Gram negative; Cefoxitin disk diffusion and PCR for presence of the mecA gene among Gram positive strains. Disc diffusion method was done to assess susceptibility to Tigecycline and Colistin. Results: Variable drug resistance 45% XDR and 23% MDR for Gram negative and 28%, 19%, respectively for Staphylococcus species was recorded. The high sensitivity to Tigacycline was found among all Klebsiella, E. Coli and Enterobacter isolates, also to all isolated Staphylococcal species, while highest colistin sensitivity was noticed in 33% of ESBL producing Enterobacter. Conclusion: Increased prevalence of MDROs. Tigacycline was highly active against MDROs rather than colistin.