Background: HCC comes in the sixth rank in all tumors that influence the human body and it is generally connected with interminable liver cirrhosis in 80% of patients. In Egypt, the rate of HCC has expanded pointedly in the last decade. It is considered as a heterogeneous illness including variable types of neoplasms, which includes distinctive profile changes in both mRNA and miRNA expression. One of them is miRNA 483-5P which was found to be up-regulated in hepatocelleular carcinoma. Objectives: To assess the diagnostic and prognostic potential of circulating miRNA 483-5p in HCC in Egyptian patients in comparison to chronic liver disease patients and healthy controls. Methodology: This study was done on 100 HCC patients, 50 chronic liver disease (CLD) due to HCV infection and 50 healthy subjects. Serum alpha fetoprotein (AFP) was measured in all subjects by Chemiluminescence. The relative quantitation of miRNA 483-5p was determined by real-time quantitative Polymerase Chain Reaction (RT-qPCR). Results: the results revealed an over expression of miRNA-483-5p in HCC group (p < 0.001) compared to both CLD and healthy subjects, while no significant change was detected between CLD and healthy subjects. MiRNA483-5p expression increased significantly with increased number of focal lesions and size of tumor. Receiver Operator of Characteristics (ROC) curve analysis of plasma miRNA-483-5p revealed that, at a cut-off value of 3. 22 (fold expression), the sensitivity and specificity for differentiation of HCC cases were 88.0% and 92.0%, respectively. Conclusion: Circulating miRNA483-5p can be used as an early diagnostic biomarker for detection of HCC.