Background: The GeneXpert MRSA/SA Blood Culture (BC) assay was validated prospectively in patients with suspected bacteremia. Objectives: The purpose of this study was to compare the Xpert MRSA/SA Blood Culture Assay to conventional culturing techniques. Methodology: Positive blood cultures flagged up by the BacT/ALERT® 3D system were gram stained and subcultured. Results: Out of the 33, 28 (84.8%) were identified as CoNs by phenotypic methods. The remaining 5 were identified as S aureus. GeneXpert results were concordant with the phenotypic results in 30 (90.9%) of samples. Twenty two of the CoNs were methicillin-resistant isolates and five were methicillin-sensitive isolates. Of the 5 S aureus , 3 were concordant. One MRSA and two MSSA. This result is statistically significant at p < .05. The mean time to notification of Xpert BC assay results was 1.5 hours. Conclusion: The Xpert assay is dependable, fast and reproducible for on-demand testing in health care settings where such testing is needed.