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285544

Evaluation of CD27 Expression on Mycobacterial Antigen-Specific CD4+ T Cells as an Immunological Marker for Diagnosis of Active Pulmonary Tuberculosis

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Last updated: 05 Jan 2025

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Abstract

Background: Until now, there is a need for novel tools helping the prompt diagnosis tuberculosis (TB). Objective: This study aims to explore the extent of CD27 expression on ESAT-6 & CFP-10 antigen-specific CD4+ T cells as a recent biomarker for rapid diagnosis of active pulmonary TB in Egypt. Methodology: Based on IFN-γ+ expression, ESAT-6 & CFP-10 antigen-specific CD4+ T cells were identified and the CD27 expression was analyzed for 60 M. tuberculosis-infected patients and 20 healthy controls. Results: The present data, in fact, displayed that patients with pulmonary TB had significantly lowered CD27 expression than healthy controls (P <0.001). The persistent active TB patients had much lesser percentages of CD27+ T cells than culture-positive recently infected TB patients (P =0.014) and healthy controls (P< 0.001). On evaluating the diagnostic performance of CD27 expression, it has been declared that at a cutoff value of 43.64%. CD27 had a sensitivity, specificity, positive predicted value (PPV), and negative predicted value (NPV) 86.7%, 100%, 100%, 71.4% respectively, for discrimination between patients with TB from healthy subjects. Conclusion: Thereby, proportion of CD27+ antigen-specific CD4 T cells could be used as an immunological marker for active TB.

DOI

10.21608/ejmm.2018.285544

Keywords

tuberculosis, Flow cytometry, CD4+ T cells, CD27, ESAT-6 & CFP-10 antigens

Authors

First Name

Mona

Last Name

Gadallah

MiddleName

Abd El-Aziz

Affiliation

Medical microbiology and immunology department,faculty of medicine,Tanta university,Egypt

Email

mona.gadallah@med.tanta.edu.eg

City

-

Orcid

-

First Name

Abo Elfetoh

Last Name

Elanany

MiddleName

E.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Tanta University

Email

-

City

-

Orcid

-

First Name

Marwa

Last Name

Abd Elwahab

MiddleName

A.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Tanta University

Email

-

City

-

Orcid

-

First Name

Basem

Last Name

El Shaffey

MiddleName

I.

Affiliation

Department of Chest diseases, Faculty of Medicine, Tanta University

Email

-

City

-

Orcid

-

First Name

Wageih

Last Name

El-Naghy

MiddleName

S.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Tanta University

Email

-

City

-

Orcid

-

First Name

Ayman

Last Name

El-dib

MiddleName

S.

Affiliation

Department of Chest diseases, Faculty of Medicine, Tanta University

Email

-

City

-

Orcid

-

First Name

Dalia

Last Name

Sharawy

MiddleName

El

Affiliation

Department of Chest diseases, Faculty of Medicine, Tanta University

Email

-

City

-

Orcid

-

First Name

Nadia

Last Name

Dahy

MiddleName

M.

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Tanta University

Email

-

City

-

Orcid

-

First Name

Mahavir

Last Name

Singh

MiddleName

-

Affiliation

Lionex Diagnostics and Therapeutics, SalzdahlumerStraße 196, 38126 Braunschweig, Germany

Email

-

City

-

Orcid

-

First Name

LIONEX

Last Name

Scientific Research Group

MiddleName

-

Affiliation

Lionex Diagnostics and Therapeutics, SalzdahlumerStraße 196, 38126 Braunschweig, Germany

Email

-

City

-

Orcid

-

Volume

27

Article Issue

2

Related Issue

39632

Issue Date

2018-04-01

Receive Date

2023-02-14

Publish Date

2018-04-01

Page Start

71

Page End

79

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 27_Issue 2_Pages 71-79.pdf

PDF . 619.3kB

Link

https://ejmm.journals.ekb.eg/article_285544.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=285544

Order

285,544

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Evaluation of CD27 Expression on Mycobacterial Antigen-Specific CD4+ T Cells as an Immunological Marker for Diagnosis of Active Pulmonary Tuberculosis

Details

Type

Article

Created At

28 Dec 2024