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283355

Role of HLA-G 14 bp polymorphism and soluble HLA-G level in recurrent spontaneous abortion

Article

Last updated: 05 Jan 2025

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Abstract

Background: The immune response of the mother against her embryo is supposed to be responsible for about 50 % of recurrent spontaneous abortion (RSA) case. Objectives: To assess the prevalence of HLA-G 14bp insertion/deletion (ins/del) polymorphism in females with RSA and normal pregnant females and compare the plasma levels of soluble HLA-G (sHLA-G) in the studying groups. Also, we intended to explore the association between HLA-G 14 bp polymorphism and sHLA-G plasma levels. Methodology: This case-control study involved 50 females with RSA and 50 control pregnant females. The genotype for HLA-G 14 bp (ins/del) polymorphism was performed by PCR and their sHLA-G plasma levels were measured. Results: The HLA-G del/del genotype and ins/ins genotype frequencies were significantly different in RSA group as compared to controls (P=0.002). Additionally, the incidence of the 14-bp ins allele was significantly raised in RSA group than in control (67 vs. 41%, respectively; P=0.0004). Consequently, the higher frequency of 14-bp ins allele in RSA group as compared to controls indicate that this allele is associated with an increased risk of RSA (OR 2.9, 95% CI: 1.6-5.2, P=0.0003). Conclusion: The plasma level of sHLA-G is a promising marker in the management of RSA and could be an early indicator of the fate of In Vitro Fertilization (IVF). The polymorphism in the HLA-G gene, specifically, in the 3′UTR region of exon 8 affects the plasma level of sHLA-G and subsequent pregnancy outcome.

DOI

10.21608/ejmm.2019.283355

Keywords

Recurrent spontaneous loss, HLA-G 14bp in/del, exon 8, HLA-G gene, HLA-G genotype

Authors

First Name

Alia

Last Name

El Shahaway

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

aliaabdelmonem@yahoo.com

City

-

Orcid

-

First Name

Wafaa

Last Name

Salah

MiddleName

-

Affiliation

Obstetrics and gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

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City

-

Orcid

-

First Name

Sherin

Last Name

Shazly

MiddleName

A.

Affiliation

Obstetrics and gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

-

City

-

Orcid

-

First Name

Rasha

Last Name

Abd Elhady

MiddleName

R.

Affiliation

Obstetrics and gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Email

-

City

-

Orcid

-

Volume

28

Article Issue

4

Related Issue

39292

Issue Date

2019-10-01

Receive Date

2023-01-31

Publish Date

2019-10-01

Page Start

127

Page End

132

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_283355.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=283355

Order

283,355

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Role of HLA-G 14 bp polymorphism and soluble HLA-G level in recurrent spontaneous abortion

Details

Type

Article

Created At

28 Dec 2024