Background: Helicobacter pylori (H.pylori) infection is a chronic bacterial infection affecting the gastric mucosa leading to wide spectrum of gastrointestinal disorders ranging from chronic gastritis and peptic ulcer disease to gastric carcinoma. Objectives: This study was performed to describe endoscopic features and microbiological findings of H.pylori related dyspepsia among dyspeptic patients attending Gastrointestinal Tract Endoscopy Unit in Suez Canal University Hospital. Methodology: This single-center, cross-sectional descriptive study recruited all patients, aged over 18 years of both sex with dyspeptic symptoms who agreed for endoscopic evaluation. Gastric biopsy samples were obtained; histological examination and culture were performed for every patient with Rapid Urease Test (RUT) positive sample. Results: Out of 95 adult patients with dyspeptic symptoms underwent upper endoscopy after giving consent, 96.8% were diagnosed to have H.pylori infection using histological examination while only 7.4% of them were positive for H. pylori by culture. Conclusion: There is a high prevalence of H.pylori associated dyspepsia. Culture has a little yield in diagnosing H.pylori infection compared to histological examination.