Background: A great alertness should be paid for the alarming rate of multi-drug resistance (MDR) in Pseudomonas aeruginosa (P.aeruginosa); the third most prevalent pathogen correlated to nosocomial catheter-associated urinary tract infections (CAUTIs), making traditional treatment much more difficult. Nanoparticles represent a promising alternative. Objective: To assess the antibacterial effect of silver nanoparticles (AgNPs) against MDR P. aeruginosa. Methodology: Minimum inhibitory concentrations (MICs) of AgNPs evaluated using broth microdilution assay and minimum bactericidal concentrations (MBC) determined by the lowest concentrations required to kill 99.9% of the initial inoculum. Results: The MIC and MBC values ranged from 1 to 8 μg/ml and 2 to 32 μg/ml, respectively. Conclusion: Obtained results were very promising suggesting that AgNPs exhibited considerable bacteriostatic and bactericidal effect, thus may be considered as an effective solution in fighting against MDR P.aeruginosa.