Background: Pseudomonas aeruginosa have various virulence factors which participate in the bacterial invasion and toxicity. Different genes are responsible for their toxicity such as toxA, algD and plcN, exoS, lasB and plcH gene. Objectives: This work aimed to isolate and identify Pseudomonas aeruginosa that are prevalent in burn and Plastic Surgery Departments at Benha Teaching Hospital and to detect exoS, lasB and plcH virulence genes. Methodology: This work was done on 100 patients who had sustained burn injury from outpatients and inpatients of Benha Teaching Burn and Plastic Surgery Departments during the period from November 2016 to the end of April 2017. Burn wound samples were subjected to isolation and identification, exoS, lasB and plcH genes from 33 of Pseudomonas aeruginosa isolates were detected by using multiplex PCR. Results: this work showed that the most common cause of burn wound infections were Pseudomonas aeruginosa (44.7%). Among the 33 Pseudomonas aeruginosa isolates it was found that 26 (78.8%) of isolates have las B gene, 23 (69.7%) of isolates have plc H gene and 14 (42.4%) of isolates have exo S gene while the three genes were found in only 10 (30.3%) of isolates. Conclusion: : High percentage of isolated P. aeruginosa (78.8%) contains lasB genes This finding shows that P. aeruginosa was pathogenic , virulence and play an important role in burn infections