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283193

New Method for Diagnosis of Salmonella Gastroenteritis in Pediatric Patients

Article

Last updated: 05 Jan 2025

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Abstract

Background: Non- typhoidal salmonella infection is a major cause for acute bacterial gastroenteritis in children. There is no ideal method for diagnosis of salmonella infection and the development of highly selective media continues to be of particular importance for diagnostic and treatment purposes. Objective: Was to assess the performance of two media, the first one was the MSRV agar as a screening medium for isolation of salmonella from stool samples of pediatric patients with gastroenteritis and the second medium was the new salmonella single tube to identify Salmonella spp. Methodology: Stool samples were subjected to stool analysis, isolation of Salmonella species on selective culture media (XLD, SS & MSRV). Different biochemical reactions were used to identify Salmonella spp; the first was the conventional biochemical reactions and the second was the newly suggested salmonella single tube. Conventional PCR was done to detect the genus salmonella and the serovars enteritidis and typhimurium. Results: Comparing the results of the three selective culture media, the validity values observed with MSRV medium were superior to those obtained by the other two media. Salmonella single tube yielded 100% sensitivity, specificity, positive predictive and negative predictive values with reliability equivalent to that of the conventional 5 tubes method. Salmonella typhimurium was prevalent in 75% of positive patients while Salmonella enteritidis was detected in 25%. Conclusion: When MSRV agar was evaluated in comparison to other selective media, it had the highest values in terms of performance. Direct plating of MSRV agar produced the same performance of indirect plating therefore, direct plating could shorten the detection time of Salmonella spp. Using salmonella single tube ensures simple and rapid processing in one step and is very suitable for mass screening purposes.

DOI

10.21608/ejmm.2019.283193

Keywords

Non, typhoidal salmonella, gastroenteritis, MSRV agar, Salmonella single tube

Authors

First Name

Sahar

Last Name

Fayed

MiddleName

Mohamad

Affiliation

Department of Clinical and Chemical Pathology, Faculty of Medicine, Banha University, Egypt

Email

saharmohamadfayed@gmail.com

City

egypt

Orcid

-

First Name

Doaa

Last Name

Soliman

MiddleName

R.

Affiliation

Department of Pediatrics, Faculty of Medicine, Banha University, Egypt

Email

-

City

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Orcid

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Volume

28

Article Issue

4

Related Issue

39292

Issue Date

2019-10-01

Receive Date

2023-01-30

Publish Date

2019-10-01

Page Start

1

Page End

8

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_283193.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=283193

Order

283,193

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

New Method for Diagnosis of Salmonella Gastroenteritis in Pediatric Patients

Details

Type

Article

Created At

28 Dec 2024