Background: Non-fermenting Gram-negative rods (NFGNRs) are widely distributed in nature. Objective: The study was done to determine the prevalence of NFGNRs in Suez Canal University hospitals (SCUH). Methodology: During a one year period, batteries of 370 specimens were processed and subjected to biochemical reactions. Results: Among the total 305 pathogenic specimens, only 46 specimens (15%) were identified as NFGNRs. Pseudomonas aeruginosa was the commonest isolate, accounting for 45.6% of NFGNRs, followed by Acinetobacter baumannii 17.4 %. Antimicrobial susceptibility tests of NFGNRs isolates were performed and it was found that the majority were multi-drug resistant (MDR). Conclusion: Therefore, NFGNRs represent a considerable health problem in SCUH.