Background: Urinary Tract Infection (UTI) is one of the most common bacterial infections both in community and hospital settings. Urine samples constitute a major proportion of the samples tested in routine diagnostic laboratories. Fosfomycin used to treat UTI for both gram positive and negative bacteria. The aim of the study was to evaluate the resistance of some uropathogens to fosfomycin because antibiotic resistance is growing with time, so continuous evaluation of antibiotic is urgent.
Materials and methods: Mid-stream urine samples were collected from patients suspected with UTI. All these samples cultured aerobically at 37°C for overnight. A total of 100 bacteria of both E. coli and gram positive cocci were diagnosed by general diagnostic bacteriological methods. Disc diffusion method used for antibiotic sensitivity test; two discs were tested, fosfomycin 200 µg and Amoxyclav 30 µg. All the statistical analysis was done by using SPSS 26 software and Excel app.
Results: From the total of 100 positive specimens, female was high prevalence with 70 specimens (70%), while males were just 30 specimens (30%) significantly (P< 0.0001). fosfomycin in E. coli showed high sensitivity rate 87.5% compared to Gram positive cocci (G+ ve cocci) that was 100 % sensitive to fosfomycin with significant differences (P= 0.02). For Amoxyclav, both E. coli and Gram positive cocci revealed different rate of sensitivity 31.3 % and 66.7 % respectively with significant differences (P= 0.001).
Conclusion: Fosfomycin is effective in both E. coli and G+ ve cocci, but it is  more effective against G+ ve cocci 100% than gram negative bacteria (E. coli) 87.5% significantly (P=0.02). Amoxyclav showed high activity against G+ ve cocci 66.7% compared to E. coli 31.3% significantly (P= 0.001).