Bovine viral diarrhea (BVD) is one of the most imperative worldwide diseases in ruminants leading to substantial damage as well as extensive economic losses for the cattle industry. there have been no reports about the disease's situation in New valley province, so the goal of this study was to estimate the level of bovine viral diarrhea virus (BVDV) infection in cattle herds through serodiagnosis and to determine factors influencing the course of infection (current epidemiological situation) which will aid in establishment of control strategy. In this study a total of 1400 cattle of different ages, sexes and breeds (not previously vaccinated with BVD vaccines) were screened clinically for symptoms of BVD infection. 260 cattle out of totally 1400 screened cattle (18.6%) showing clinical signs arousing suspicion of BVD (49 cattle (18.8%) exhibit respiratory manifestation, 63 (24.2%) cattle suffered from diarrhea, 37 (14.2%) with mixed enteric and respiratory signs , 43 (16.5%) with retarded growth, oral lesion was detected in only 6 cattle (2.3%) , While the congenital anomalies were recorded in 18 (6.9%) , abortion occurred in 29 female cattle (11.1%) and only 15 cattle ( 5.8%) suffered from infertility trouble ) .Totally 260 clinically diseased cattle undergo Serum Neutralization Test (SNT) for detection of BVDV infection. The results revealed that 83 cattle out of 260 (31.9%) were detected as seropositive (positive for BVDV antibodies).