The purpose of this study was to investigate the occurrence of non-typhoidal Salmonella (NTS) and the distributed serotypes in New Valley and Assiut provinces. Eight hundred samples were collected (280 from New Valley and 520 from Assiut) from different sources including fecal swabs (160), raw milk (80), Damietta cheese (80), Kareish cheese (80), ice cream (80), yoghurt (80), stool samples (170) and hand swabs (70). Samples were bacteriologically examined then the recovered isolates were serotyped. In New Valley province, occurrence of NTS was 8.57%, 3.33%, 0.00%, 20,00%, 17.14% and 5.71% of milk, Kareish cheese, Damietta cheese, yoghurt, fecal swabs and stool samples, respectively, while, the occurrence of NTS in Assiut was 15.56%, 18.00%, 12.50%, 4.44, 8.75%, 15.56%, 11.43% and 8.00% of milk, Kareish cheese, Damietta cheese, yoghurt, ice cream, fecal swabs, hand swabs and stool samples, respectively. 13 different serotypes were detected in the recovered isolates (87) including S. typhymurium and S. enteritidis were the predominant serotypes with an occurrence rate of 24.14% and 20.69%, respectively, followed by S. tsieve (12.64%), S. infantis (8.05%), S. larochelle (9.20%), S. virchow (5.75%), S. molade (8.05%), S. haifa (3.45%), S. shubra (1.15%), S. alfort (1.15%), S. essen (2.30%), S. apeyeme (1.15%) and S. heidelberg (2.30%). In conclusion, the high prevalence of the NTS in various dairy products represents an alarm for the public health because of the possibility of transmitting these serovars to consumers, which requires reviewing the methods of control and prevention.