The present investigation was carried out to study the prolactin and growth hormones genes expression, interleukin (IL-1β) and Transforming growth factors (TGFβ-1a) genes expression of Nile tilapia (Oreochromis niloticus). Three groups; control (not transported), post transport with no salt and post transport with addition of the salt in an extremely 5-hour transport model were used. Inclusively, the studied parameters were intensely varied in the post transport withno salt fish group than post transport with salt fish group. PRL188 gene was significantly down regulated in the post-transport with no salt (0.67) and post-transport with the salt (0.88) fish groups. GH gene expression was significantlydown regulated in the post-transport with no salt (0.37) and post-transport with salt (0.79) groups. IL-1β was up-regulated to 2.27 folds in the post-transport with no salt group and to 1.15 folds in the post-transport with the saltgroup and TGFβ-1a was also up-regulated to 9.65 folds in the post-transport with no salt and to 4.32 folds in the post-transport with the salt group. All results revealed that the transport has bad effects on the skin and the gene expressionof growth and prolactin hormones, while the addition of 5g NaCl/L transport water mitigated the bad effect of transportmoderately and preserved the surface skin features of transported O. niloticus.