Background: Organizational zero tolerance policies are essential in promoting ethical behavior and creating a safe work environment, particularly and reduce performance obstacles in healthcare settings. Aim: this study aimed to assess the relationship between organizational zero tolerance policy and performance obstacles among staff nurses. Research Design: Comparative correlational research design was used in this study. Setting: The study was conducted at two public Hospitals (Bilbais governmental hospital affiliated to Ministry of Health and Sadnawy hospitals affiliated to the educational hospitals). Sample: A convenient sample of staff nurses at the time of study was utilized in this study. Tools: Two tools were used, zero tolerance policy questionnaire and Performance obstacles questionnaire. Results: the study illustrated that there was a highly statistically significant negative strong correlation between performance obstacles and zero tolerance policy Conclusions: this study conducting that There was a highly statistically significant negative correlation between zero tolerance policy and performance among the studied staff nurses. Recommendations: Regularly review and update the zero tolerance policies to ensure that they are effectively reducing performance obstacles in healthcare setting.