Background: Sickle cell anemia is a hereditary blood disorder characterized by the production of abnormal hemoglobin. Children with sickle cell anemia suffer from chronic pain, frequent infections, and fatigue due to the blockage of blood flow and the subsequent reduction in oxygen delivery to tissues. Aim: This study aimed to evaluate the effect of health educational program for mothers having children suffering from sickle cell anemia. Research design: A quasi-experimental research design was used in this study. Sample: Purposive sample include 150 mothers with children suffering from sickle cell anemia. Setting: It was conducted at hematology outpatient clinic at Baharia Oasis hospital in Baharia Oasis City. Tools: One tool included four parts: 1st: Demographic characteristics of mother and child, 2nd: Medical history for the child, 3rd: Mothers' knowledge about sickle cell anemia, and 4th: Mothers' reported practice about caring of children with sickle cell anemia. Results: The study result revealed that, 5.3 % of studied mother had good total knowledge pre apply education program become 77.2 % of them had good total knowledge post apply education program. While, 4.9 % of studied mother had satisfactory with total reported practices pre apply education program, become 98.0 % of them had satisfactory total reported practices post apply educational program. Conclusion: The study concluded the health education program improved the knowledge, and reported practices of mothers about caring of children with sickle cell anemia. Recommendations: Continuing health education program for mothers with children suffer from sickle cell anemia to improve their condition.