Background: blood donation is a vital procedure of worldwide healthcare and a form of therapeutic phlebotomy as a primary medical intervention. So, it requires attention from health care providers to increase awareness regarding it especially among youth. Aim: the study aimed to assess nurses knowledge, practice and attitude regarding blood donation measures at Menoufia University. Design: A descriptive research design was used in this study that was conducted in Faculty of Commerce and Faculty of Law at Menoufia University. Subject A multi-stage random sample of 350 students. Tools: 2 tools were used, tool 1: Structural Interviewing Questionnaire including four parts: Part I: Students` demographic characteristics. Part II: Students' blood donation history. Part III: Students' knowledge about blood donation measures. Part IV: Students' reported practices measures. Tool II: University students` blood donation attitude scale. Results: 65.7% of students had unsatisfactory level of total knowledge regarding blood donation measures. Also, 57.7% of students had inadequate total reported practices regarding blood donation measures. In addition, 52.0% of the studied students had negative attitude toward blood donation measures. Conclusion: less than two thirds of studied students had unsatisfactory level of total knowledge, more than half of them had negative attitude and more than two thirds of them had inadequate reported practices regarding blood donation. In addition, there was highly statistically significant positive correlation between total knowledge, attitude and reported practices of studied students regarding blood donation. Recommendation: Implementing health educational programs to improve knowledge, attitude, and safety practices regarding blood donation among university students.