Background: Green human resources management is the alignment of established human resource practices with environmental goals and policies. It aims to achieve the environmental objectives of the organization, increase efficiency, reduce costs, and attract the best employees who are aware of sustainability processes.Aim: To assess challenges and barriers of green human resources management in healthcare as perceived by nurse managers. Design: A descriptive research design was used. Setting: The study was conducted at Elaraby hospital which is located at Ashmoun, Menoufia governorate. Subjects: The study subjects included all available nurse managers (N=50). Tools: Two tools were used for data collection, Tool 1 : Green Human Resource Management Knowledge Questionnaire, Tool 2: Perceived Challenges and Barriers by Nurse Managers about Green Human Resource Management Questionnaire. Results: The majority of nurse managers had unsatisfactory level of knowledge about green human resource management. Also, Less than two thirds of nurse managers had positive perception about challenges and barriers of green human resource management. Finally, there was no statistically significant correlation between total perception and total knowledge about green human resources management among nurse managers. Conclusion: The highest percentage of nurse managers had a positive perception about challenges and barriers of green human resources management. Recommendation: Implement green human resources management policies and practices within the healthcare organization, integrate environmental sustainability goals into the organization's vision, mission and values. In addition, integrate environmental sustainability topics into nursing education curriculum to increase nursing students awareness about green management and environmental sustainability.