Hearing loss in newborns poses a significant challenge to their development, emphasizing the need for robust newborn hearing screening test. Maternal and Child Health Centers serve as ideal settings for implementing these test. Aim of the study: To assess nurse's awareness regarding newborn hearing screening test at maternal and child health centers in Kufr El-Sheikh City. Research design: A descriptive research design was used in this study. Setting: The study was conducted in the maternal and child health centers in Kufr El-Sheikh City. Sample: Convenience sampling technique was used: to choose 186 nurses. Tools: Two tools used: 1st tool is structured Interviewing questionnaire. It consist of 3 parts: 1) demographic characteristics, assessment of nurse's knowledge, and assessment of nurse's attitude. 2nd tool is observation Assessment of nurse's practice regarding newborn hearing screening test. Results: The study result revealed that, 65.1% of the studied sample had unsatisfactory level of total knowledge, 50.5% of the studied sample had negative attitude, 70.4% of the studied sample had adequate practice regarding newborn hearing screening test. Conclusion: Deficit in nurses' knowledge regarding newborn hearing screening test . Furthermore, there were highly statistically significant positive correlation between total scores of knowledge, total scores of attitude and total score practice of the studied sample regarding newborn hearing screening test at (P= < 0.01). Recommendation: Training programs should be developed and implemented for nurses to increase their awareness about newborn hearing impairment and screening test.