Background: EMR systems are now necessary. It will be of great assistance in converting the health care system from manual, paper-based processes to computerized, online ones. It will assist in delivering high accuracy, timeliness, and efficiency while using clinical information and help nurses do their jobs more effectively. The present study aims to determine the effect of applying an electronic medical record system for nurses on patient safety culture. Design: a quasi-experimental research design was used. Setting: the study was conducted in Intensive Care Units (ICU) at National Cancer Institute (NCI), Cairo University. National Cancer Institute. Sample: a purposeful sample composed of (50) ICU nursing personal. Tools: data was collected using nurse's knowledge questionnaire sheet, patient safety culture and evaluation of electronic medical record. Results: high statistically significant differences regarding ICU nurse's knowledge, effect in patient safety cultures and nurse satisfaction pre, post ant three months post implementation of the program with P value (0.000). Conclusion: All ICU nurses who attended the designed training program showed a relative improvement in knowledge and perception during post and follow-up phase as compared to preprogram phase which supported the study hypothesis. Recommendations: Providing adequate computer training can make the transition process easier for users wary of change and will ensure that your entire team is ready use EMR and a durable record of patients' healthcare is provided through EMRs with the usage of different procedures to reduce vulnerabilities and solve problems in the modernized healthcare medical records.