Background: Virtual learning environment a self-contained computer based online enabling interactions between instructor and learner on the same time. Aim: Was to assess challenges and benefits of using virtual learning environment as perceived by nurse educators and students. Design: A descriptive design was used in this study. Setting: This study was conducted at nursing technical institutions of the General Authority for Hospitals and Educational Institutes in Cairo and Giza- Egypt that contains eight nursing technical institutes. Subjects: All nurse educators who were worked in the technical institutes (N=40) and all students in the second year (N=200) .Tools: Three tools were used for data collection: Nurse Student knowledge about virtual learning, Nurse student's perception about virtual learning questionnaire and Nurse educator's perception about virtual learning environment questionnaire. Results: 66% of nurse students had unsatisfactory level of total knowledge about virtual learning environment, 82.5% & 77.5% of nurse educators had a negative perception about time and resources and communication challenges respectively. Regarding benefits of virtual learning environment 85% of them had a positive perception. Meanwhile, 75.5% &72.5% of nurse students had a negative perception about time and resources and communication challenges respectively. While, 80.5% of them had a positive perception about benefits of virtual learning environment. Conclusion: The highest percentage of nurse students had unsatisfactory level of knowledge about virtual learning. Additionally, 57.5% of nurse educators and 56% of nurse students had negative perception about virtual learning. Recommendation: Provide courses at educational institutions about virtual learning for both nurse educators and students.