Plant parasitic nematodes are harmful agricultural pests, causing severe yield losses of a wide range of crops worldwide. The lack of effective nematode management products has increased demand for innovative nematode management tools. This work was conducted to test propolis (bee glue) at different concentrations to control the root-knot nematode (Meloidogyne spp.) under laboratory conditions. A sample of propolis was measured and ethanol solvent (95%) was added and kept at room temperature for 24 h. The nematode eggs were exposed to the propolis extract at different concentrations (i.e., 2000, 4000, and 6000 ppm) for 24, 48, and 72 h. Results showed that propolis extract caused significant decreases in egg hatching, but to varying degrees. There was a gradual decrease in egg hatching with increasing the extract concentration and the duration of exposure.