This investigation was carried out at Etay El-Baroud Agricultural Research Station farm, El-Beheira Governorate during two winter seasons of 2021/2022 and 2022/2023, to explore new genotypes more resistant to chocolate spot disease than the local cultivars and clearing the correlation between the yield productivity and physiological traits. Eight new diverse faba bean genotypes (GB6, GB11, GB12, GB13, GB16, GB17 GB18 and GB20) and six faba bean cultivars (Sakha 1, Sakha 4, Giza716, Giza 843, Misr 1, and Giza 40) were used in this study. Genotypes were arranged in randomized complete block design with three replicates. The new genotypes GB 16, GB17, GB18 and GB20 were more resistant to chocolate spot disease under both of artificial infection with Botrytis fabae virulent isolate (B. f N) and under natural infection in the open field. These genotypes possessed high mean values for yield and its components criteria in the second season only. The resistant new genotypes (GB 17 and GB 20) have surpassed the resistant cultivars (Sakha 1 and Sakha 4) in photosynthetic pigments content (Chlorophyll a, b). There was a positive correlation between decreasing chocolate spot disease severity and the increasing of total phenols, peroxidase activity and proline content. The two-way hierarchical cluster analysis showed that disease severity, number of pods plant-1, seed yield ardab fad-1, Chl a, Total Chl., peroxidase and shoot dry weight after 70 days are related characters.