Evaluation of genotypes in arid and semi-arid regions comes in the first order for Sugar beet Breeding Program of Desert Research Center (DRC) due to its importance in determining this adaptation under stress conditions, especially after the success of flowering and seed production experiments of sugar beet under natural conditions of Saint Catherine. So, we need to optimize genotypes production by evaluation and adaptation under Egyptian conditions. A comparative study was conducted to assess the performance of twelve sugar beet genotypes in three salinity levels (L1, L2 and L3) in Ras Sudr station, South Sinai, DRC during 2016/2017-2017/2018 seasons. The experimental design was randomized complete block design with three replicates. The results indicated that the interaction between years, salinity and sugar beet genotypes mean squares were highly significant for all traits. This interaction between environmental conditions and sugar beet genotypes will have a significant impact on future breeding programs. HM16578 genotype gave the highest values of leaves weight per plant (205.8g, 200.4g and 111.7g) in L1, L2 and L3, respectively. DE 034-665 genotype recorded the highest values of root length; root diameter and root weigh per plant in L1and L2, while DE154-7682-118 genotype gave the highest values in L3. Primera genotype recorded the highest values of total soluble solids percentage (24.33, 26.00 and 27.67%) in L1, L2 and L3, respectively. Total soluble solids percentage showed a high significant negative correlation with each of leaves weight per plant, root length, root diameter and root weight per plant.