The present study was carried out during two successive seasons (2016 and 2017) at El-Farafra Oasis region, New valley governorate, Egypt to study the effect of different pollen grain sources (locations) on productivity and fruit quality of Sewi date palm. Female Sewi date palms of healthy, nearly homogenous in growth as well as fruiting ability were selected. The selected Sewi palms were subjected to all horticultural practices applied to the date palms in this region, except those ones under study. The number of female inflorescences/ palm was adjusted to 8 by removing excess earliest, latest, and small clusters. In addition, male date palms as a pollen sources were selected from five locations (Wahat Bahariya, Farafra, Fayoum, Qaliubiya and Giza). All male date palms were selected and marked to complete investigation in both studied seasons. Pollen grains samples were collected at flowering time (beginning of March) from five locations and prepared for pollination. The prepared pollens of each source were used for pollinating 15 female date palms Sewi cv. by using hand pollination (manual method) during March and April in both seasons of this study to detect the effect of pollen grains source on productivity and fruit quality. The most important results can be summarized as follows: Wahat Bahariya location surpassed significantly other males as it gave the highest weight, length and width of spathe during 2016 and 2017 seasons. In addition, Wahat Bahariya location resulted the heaviest and tallest male inflorescence with the tallest strands with also the greatest number of strands per inflorescence during 2016 and 2017 seasons. Moreover, the greatest values of pollen grains viability were significantly coupled with Fayoum location during 2016 and 2017 seasons. Additionally, Farafra and Wahat Bahariya locations ranked statistically second during the two seasons. The highest fruit weight of female Sewi date palm was recorded when using pollen grains from Fayoum location during both experimental seasons. The highest values of fruit length, diameter, shape index, fruit pulp weight (g) and fruit pulp (%) were contaminant with Giza location pollen source in the two seasons. The highest values of total sugars, reducing sugars and T.S.S. percentages were statistically in concomitant to Farafra and Wahat Bahariya location pollen sources in the two seasons.