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344255

GENETIC RELATIONSHIPS AMONG TEN SUNFLOWER INBRED LINES BASED ON ISSR AND RAPD ANALYSES

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Last updated: 28 Dec 2024

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Abstract

The present investigation was conducted to access the genetic diversity among ten inbred lines of sunflower: Ha89, Ha93, Ha64, Ha101, Ha122, Sha13, Sha14, Sha15, Nsha136 and Nsha140 imported from different origins using RAPD and ISSR techniques. A total of 95 major scorable bands ranging from 117 to 1075 bp were generated from 8 ISSR primers showing 78.8% polymorphism. While, a total of 150 scorable bands ranging from 129 to 2835 bp were produced from 10 RAPD primers detecting 91.3 % polymorphism. Unique bands were produced by both ISSRs and RAPDs. Genetic similarity values were ranged between 51.1- 84.1 % and 38.9- 82.4 % for ISSR and RAPD, respectively. The lowest similarity using ISSR primers was observed between Sha14 and Ha93. While, the lowest similarity using RAPD primers was found between Ha89 and Nsha140. The dendrogram based on ISSR data separated the ten inbred lines into two main clusters at a similarity coefficient of 0.25, while, the dendrogram related to RAPD data separated them into three main clusters. The combined data of RAPD and ISSR showed genetic similarity ranged from 51.0 to 77.8 %. Also, the dendrogram based on the combined data of both ISSR and RAPD displayed considerably similar results to those obtained from individual ISSR analysis.

DOI

10.12816/ejpb.2019.344255

Keywords

Sunflower, Helianthus annuus L. Inbred line, molecular markers, Genetic diversity, ISSR and RAPD

Volume

23

Article Issue

4

Related Issue

46432

Issue Date

2019-04-01

Receive Date

2024-03-03

Publish Date

2019-04-01

Page Start

547

Page End

563

Print ISSN

1110-7863

Online ISSN

2735-3885

Article File

EJPB_Volume 23_Issue 4_Pages 547-563.pdf

PDF . 772.5kB

Link

https://ejpb.journals.ekb.eg/article_344255.html

Detail API

https://ejpb.journals.ekb.eg/service?article_code=344255

Order

344,255

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Original research

Type Code

1,909

Publication Type

Journal

Publication Title

Egyptian Journal of Plant Breeding

Publication Link

https://ejpb.journals.ekb.eg/

MainTitle

GENETIC RELATIONSHIPS AMONG TEN SUNFLOWER INBRED LINES BASED ON ISSR AND RAPD ANALYSES

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Type

Article

Created At

28 Dec 2024