Egyptian agronomist strieved his best to increase yield via biofertilizers with less amount of mineral fertilizer. Thus a field experiments was carried out in a split plot design with randomized complete block design arrangement in three replications at Nubaria Agric. Res. Station, ARC, Nubaria region, Egypt (30° 66'N latitude and 30° 06' E longitude with an altitude of 15.00 meters above sea level), during two successive growing seasons (2019/202 and 2020/2021). The objective of this study was to investigate the effect of three nitrogen levels with a biofertilizer and 90 kg N/fed without the bifertilizer on yield and its components of Giza 171, Misr3, Misr1 and Sakha 95 wheat cultivars to maximize wheat productivity and minimize environmental pollution due to the use of mineral fertilization. The addition of the inoculum and four nitrogen levels (30 + B, 60 + B, 90 + B and 90 kg N/fed). Results showed superiority increases in yield and yield components in seasons under combined of 60 kg N + B and 90kg N + B treatments compared to 90 kg N only without the biofertilizer. Significant variations were recorded between the tested biofertilizer treatments for yield, yield components and their interaction of some characters in both seasons and combined data, except 1000- KW in the second season. Wheat cultivar, Giza 171 was the earliest cultivar, while was Sakha 95 the shortest cultivar. Also, Misr1 gave the highest number of spikes/m2 while the least was Giza 171. Wheat cultivar Misr3 was superior in numbers of kernels/ spike and 1000- kernel weight. On the other hand, Sakha 95 had the highest grain yield ard/fed. Fertilizer combination 60kg N + B gave the shortest plants and the highest number of kernels/spikes, 1000- KW and grain yield ard/fed. Wheat cultivars responded positively under 60 kg N + biofertilizer for number of spikes/m2.