The present study aimed to investigate the effects of using nanoparticles (silver, SNaPs and graphene, GNaPs) as a supplementation in broiler diets on productive performance and microbial load in the small intestine (SI) and ceca of broiler chicks. A total of 360 one-day-old Indian River sex-mixed broiler chicks were used in nine treatments with eight replicates of five chicks per replicate. The experimental rations were prepared using 0.0, 2.5, 5.0, 7.5, and 10.0 ppm/ kg feed of two types of nanoparticles (silver and graphene). All diets were formulated to meet NRC minimal nutritional requirements. Data were analyzed using a randomized complete block design in factorial arrangement with types of nanoparticles and supplementation levels of nanoparticles as main effects. The results were as follows: Productive performance including LBW, BWG, FI, PI, and EPEF differed significantly within treatments concerning the different levels of nanoparticles but wasn't affected by different types of nanoparticles during the overall trial period, also FCR wasn't differed significantly by different types or levels of nanoparticles at overall period. The count of Lactobacillus ssp. in both SI and Ceca was affected significantly by different types and levels of nanoparticles. In the same way, E. Coli in both SI and Ceca decreased significantly with increasing nanoparticle levels, while it wasn't affected significantly by different types of nanoparticles in SI it was affected significantly by different types of nanoparticles in Ceca. It can be concluded that there weren't significant differences between the two types of nanoparticles as feed additives on productive performance but microbiota in the digestive tract was affected significantly where GNaPs were better than SNaPs.