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346222

variables of cryopreserved rabbit spermatozoa as affected by nano-curcumin supplementation into Tris-extender containing egg yolk or soybean lecithin extender

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

Animal production.

Abstract

This study aimed to assess the impacts of curcumin nano-formulation (CM-NF), type of extender, and their interaction on physical characteristics of cryopreserved rabbit spermatozoa. Ten mature NZW rabbit bucks (6.6–7.8 mo old and 3.45-3.95 kg LBW) were used as semen donors. Pooled semen was diluted with Tris-egg yolk (TEY) or Tris-soybean lecithin (TSBL) extender with CM-NF (0, 1, 1.5, and 2 µg/mL), equilibrated (2h at 5°C), loaded in straws, stored in liquid nitrogen, thawed at 37oC for 30 s, and evaluated for progressive motility (PM), livability (LS), abnormality (SA), membrane integrity (MI), and acrosome integrity after dilution, equilibration, and thawing. Results showed that extender type had non-significant effect on all sperm parameters after all cryopreservation processes. In both extenders, CM-NF (1 µg/mL) recorded significantly the highest PM, LS, MI, and AI, and the lowest SA percentages compared with free-extender. Impact of CM-NF (1.5 µg/mL) on all sperm parameters did not differ from that of free-extender. Increasing CM-NF level to 2 µg/mL adversely affect PM, LS, and SA only in post-diluted semen, and had no effects on all sperm characteristics after equilibration and thawing. The interaction effect of extender type with CM-NF level on all sperm was not significant. Conclusively, there were no differences between TEY and TSBL extenders in maintaining rabbit sperm characteristics after cryopreservation. Supplementation of either TEY or TSBL extenders with CM-NF (1.5 µg/mL) can improve the progressive motility, livability, membrane integrity and acrosome integrity in cryopreserved rabbit semen.

DOI

10.21608/jsaes.2024.254166.1068

Keywords

Rabbit, Semen, protectant, nano-particles, cryopreservation

Authors

First Name

Khaled

Last Name

Abdel-Khalek

MiddleName

-

Affiliation

Animal Production Department, Faculty of Agriculture, Tanta University, Egypt

Email

abdelkhalk2004@yahoo.com

City

-

Orcid

-

First Name

Sherif

Last Name

Gabr

MiddleName

-

Affiliation

-

Email

sherif.gabr1@agr.tanta.edu.eg

City

-

Orcid

-

First Name

George

Last Name

E. Younan

MiddleName

-

Affiliation

-

Email

-

City

-

Orcid

-

First Name

Ahmad.

Last Name

M. Shehab El-Din

MiddleName

-

Affiliation

-

Email

-

City

-

Orcid

-

First Name

Aly E.B.

Last Name

Ghodaia

MiddleName

-

Affiliation

-

Email

-

City

-

Orcid

-

Volume

3

Article Issue

1

Related Issue

44652

Issue Date

2024-03-01

Receive Date

2023-12-09

Publish Date

2024-03-01

Page Start

10

Page End

17

Print ISSN

2735-4377

Online ISSN

2785-9878

Link

https://jsaes.journals.ekb.eg/article_346222.html

Detail API

https://jsaes.journals.ekb.eg/service?article_code=346222

Order

346,222

Type

Original research paper

Type Code

1,858

Publication Type

Journal

Publication Title

Journal of Sustainable Agricultural and Environmental Sciences

Publication Link

https://jsaes.journals.ekb.eg/

MainTitle

variables of cryopreserved rabbit spermatozoa as affected by nano-curcumin supplementation into Tris-extender containing egg yolk or soybean lecithin extender

Details

Type

Article

Created At

28 Dec 2024