The aim of the present work was evaluating the effect of daily oral administration of aqueous garlic extract, AGE (5 and 10 mg/kg LBW) for 30-day pre-mating on ovarian activity, and in vitro maturation and fertilization of rabbit does. Rabbit does (n=30) were allocated into three experimental groups (10/group), does in the 1st group (G1) were orally treated with distilled water (2 ml), while, those in the 2nd and 3rd groups were orally treated with 5 mg AGE/kg LBW (G2) or 10 mg AGE/kg LBW (G3) dissolved in 2 ml distilled water. Does were daily administrated before morning feeding for 30 days as a treatment period. Results indicated insignificant effect of AGE on LBW but absolute or relative ovarian weights were higher (P<0.05) by 31.6 and 36.7% in G2 and G3 compared with G1. AGE administration increased (P<0.05) large follicles number, yield, recovery rate, and quality of oocytes, the percentage of embryos at morula stages.
Daily oral administration of 10 mg aqueous garlic extract/kg LBW pre-mating improved ovarian activity, and both in vitro maturation and fertilization of rabbit does. This can be used as a tool for in vitro embryo production in rabbits with different breeds or lines.