Pomegranate juice (PJ) contains potent antioxidant 
activity against lipid peroxidation. The current study's 
objective was to ascertain the effects of various 
pomegranate juice concentrations (2.5, 5, 7.5 and 10%) 
in a tris-based extender on physical and kinetic 
spermatozoa post-dilution, equilibration time, and 
freezing of Ossimi ram semen. Semen samples were 
taken once each week for seven weeks from five rams. 
Semen was diluted at a rate of 1:20, equilibrated for 4 
hours at 5 °C, and then was loaded into 0.25 mL straws 
before being frozen in liquid  nitrogen. By using 
spectroscopic assays, it was possible to measure the 
physical, kinetic, enzyme, and antioxidant capacities of 
spermatozoa. After thawing, the 10% pomegranate 
juice addition to the extender significantly (p 0.05) 
improved the morphology of the semen (CASA, total 
motility, progressive motility, VAP, VSL, VCL, STR 
and LIN). It is concluded that the addition of 10% 
pomegranate juice to tris-based extender enhanced 
post-thawing semen characteristics and CASA motion 
dynamics of cryopreserved Ossimi ram semen while 
also   increasing   overall   antioxidant   capacity   and 
reducing MDA concentration.