Echinococcus granulosus is an animal parasite that inhabits the small intestine of the canine family and represents the final host. Livestock and humans represent the intermediate hosts where it causes hydatidosis. Hydatidosis is a neglected zoonotic disease and global public health problem caused by the hydatid cyst larval stage of E. granulosus.  Human hydatid cyst samples were collected from December 21 to January 1, 2024. Human collection areas were distributed between government and private hospitals in Nasiriyah City, Thi-Qar Province. The district's animal samples were collected from the intermediate host (livestock) from the slaughterhouses of Nasiriyah, Shatra, and the livestock market, with the help of veterinarians in diagnosing cases and extracting samples. The results showed the concentration of (protein, glucose, triglycerides and cholesterol) from different organs in humans infected with hydatid cysts. The protein concentration level in the hydatid cyst of the human lung (42.54±2.11) was significantly higher (p ≤ 0.05) than that of the human liver and ovary. The glucose concentration in the lung hydatid cyst (73.36±3.07) was significantly higher (p ≤ 0.05) than that of the human liver and ovary. The concentration of triglycerides in the liver hydatid cyst (64.63±2.90) was significantly higher (p ≤ 0.05) than that of the lung and ovary. The cholesterol concentration in the lung hydatid cyst (23.27±2.37) was significantly higher (p ≤ 0.05) than that in the liver. The results also indicated that the level of cholesterol concentration in the human lung was higher, but not significantly, than that in the ovary.