The current research was conducted to investigate the impact of injecting hatching eggs with varying levels of plasma of local chickens. In this investigation, 180 fertile eggs were divided into three groups. The first group (P0) is the control, while the eggs in both the second(P1) and third groups (P2) were injected with 50 or 100 µl of local chicken blood plasma, respectively. The eggs were injected at the first day of incubation (0 day). After 21 days, the fertility, hatching characteristics, immunological, and physiological properties of the hatched chicks were evaluated. The results revealed no significant variations in the hatching percentage, weight of the hatched chicks, and their relative weight. the physiological blood traits revealed that the third treatment showed a significant rise in red blood cells, hematocrit value, and hemoglobin with a significant decrease in white blood cells. The third treatment showed a considerable rise in the total protein concentration due to the higher globulin protein level, which is vital for immunity, and improved antioxidant glutathione levels. The treatments had no significant effect on the relative weights of the heart, liver, and residual yolk in the intestine at one day of life. Overall, it can be concluded that injecting local chicken blood plasma did not affect hatchability. This was accompanied by an improvement in the immune status of the hatched chicks, as evidenced by a decline in the white blood cell count and a rise in globulins.