This study aimed to evaluate the impact of sulpiride and PMSG hormone treatments on estrus and the number of offspring produced in female rats. Thirty mature rats were divided into three groups, each consisting of 10 females. The first group was treated intraperitoneally with sulpiride (0.25 mg/kg), the second group with PMSG hormone (20 IU/kg), and the third group with normal saline. Treatments were administered after the onset of estrus. Following treatment, natural mating occurred. The results of the study showed no significant differences between the PMSG hormone, sulpiride, and normal saline treatment groups regarding the number of females that showed estrus and mated. However, a significant difference (P < 0.05) was observed between the PMSG treatment group and the other groups with respect to the average number of embryos, which were 128, 75, and 55, respectively. The Luteinizing Hormone (LH) concentration was significantly higher (P < 0.05) in the sulpiride-treated group (1.44 ±0.12 ng/ml) compared to the PMSG hormone (1.23 ± 0.14 ng/ml) and normal saline treatment groups (0.76 ± 0.08 ng/ml). In conclusion, the application of PMSG was more effective in inducing ovulation and increasing the average number of offspring compared to sulpiride treatment in female rats.