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367647

PHENOTYPIC AND GENETIC CHARACTERIZATION OF PASTEURELLA MULTOCIDA WITH A REFERENCE TO ITS PATHOLOGICAL CHANGES IN CALVES

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Microbiology, mycology and immunology

Abstract

This study investigated the presence of Pasteurella multocida in Egyptian dairy farm calves with respiratory symptoms. A total of 150 samples were collected aseptically from 133 nasal swabs and 17 pneumonic lung tissues of calves on private farms across different governorates. Samples were tested using three methods: standard bacteriological examination, biochemical identification, and Molecular characterization for P. multocida. The P. multocida isolates were subjected to multiplex PCR for capsule biosynthesis and 16S rRNA gene. Capsular typing revealed that 60 out of 150 samples (40%) were positive for P. multocida. All isolates confirmed as P. multocida through PCR were belonged to serogroup A. Antibiotic sensitivity testing of these isolates identified enrofloxacin as the most effective drug. The most detectable resistance gene was sul II- in examined isolates with a 100% detection rate, followed by BlaTEM and tetA genes (62.5%). Histopathological examination revealed extensive damage across various organs, such as the lungs of calves, including fibrinous exudate inside alveoli and purulent fluid in other alveoli, besides thrombosis of blood vessels in P. multocida-infected animals.

DOI

10.21608/avmj.2024.272597.1229

Keywords

Pasturella multocida: Molecular Characterization: Serotyping: Histopathology

Authors

First Name

NESMA

Last Name

RASHEED

MiddleName

-

Affiliation

Department of Pathology, Animal Health Research Institute (AHRI), Mansoura Branch, Agriculture Research Center (ARC), P.O. Box 264 Dokki, 12618 – Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

HALA M.

Last Name

ISMAIL

MiddleName

-

Affiliation

Department of Pathology, Animal Health Research Institute (AHRI), Mansoura Branch, Agriculture Research Center (ARC), P.O. Box 264 Dokki, 12618 – Giza, Egypt

Email

-

City

-

Orcid

-

First Name

MAHMOUD

Last Name

ABDELRAHMAN

MiddleName

A.

Affiliation

Department of Bacteriology, Animal Health Research Institute (AHRI) (Mansoura Branch), Agriculture Research Center (ARC), P.O. Box 264 Dokki, 12618 – Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

MARWA

Last Name

SHALABY

MiddleName

-

Affiliation

Department of Bacteriology, Animal Health Research Institute (AHRI) (Mansoura Branch), Agriculture Research Center (ARC), P.O. Box 264 Dokki, 12618 – Giza, Egypt.

Email

marwaibrahim2111@gmail.com

City

Mansoura

Orcid

-

Volume

70

Article Issue

183

Related Issue

49234

Issue Date

2024-10-01

Receive Date

2024-02-25

Publish Date

2024-10-01

Page Start

11

Page End

24

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 70_Issue 183_Pages 11-24.pdf

PDF . 1.8MB

Link

https://avmj.journals.ekb.eg/article_367647.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=367647

Order

2

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

PHENOTYPIC AND GENETIC CHARACTERIZATION OF PASTEURELLA MULTOCIDA WITH A REFERENCE TO ITS PATHOLOGICAL CHANGES IN CALVES

Details

Type

Article

Created At

28 Dec 2024