348854

MOLECULAR CHARACTERIZATION AND PHYLOGENIC ANALYSIS OF CORONAVIRUS IN DIARRHEIC CALVES USING PARTIAL SEQUENCE N GENE FROM SULAYMANIYAH, IRAQ

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

Internal veterinary medicine

Abstract

Diarrhea is one of the common causes of death in calf; it has significant economic impacts in cattle industry. Calf diarrhea is most complicated and has many factors, for example, environmental, management and nutritional. Many viruses like rotavirus, bovine viral diarrhea and corona virus play a very important role in causing diarrhea. Sixty pre-weaning calves were sampled. Samples were taken from four different places at Sulaimaniyah province (Kalar, HalbjaiShahid, Khurmal, and Sharazor) between September 2022 and March 2023.The animals were divided into 3 age groups: first group was (4–30 days), second group was (31–60 days), and third group was (61–90 days). Samples were being collected directly from diarrheic calves by rectal swab for PCR testing. A fragment of the partial N gene was amplified using the Taq DNA master mix (Addbio, Republic of Korea). Phylogenetic trees were constructed based on a partial fragment of the partial N gene. The results showed that the prevalence of bovine coronavirus was (10%) 6 out of 60 collected samples; in addition, the highest prevalence was in group 1 (4-30) day of age, while the lowest prevalence rate was in group 3 (61-90) days of age. This study reported the occurrence of Coronavirus for the first time in four different places in Sulaimaniyah province, northern Iraq, using genetic analysis.

DOI

10.21608/avmj.2024.251738.1206

Keywords

Corona virus, calf, diarrhea, Phylogenic analysis

Authors

First Name

HARDI

Last Name

MARIF

MiddleName

FATTAH

Affiliation

Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani.

Email

hardi.marif@univsul.edu.iq

City

Sulaymaniyah

Orcid

0000-0002-8940-396X

Volume

70

Article Issue

181

Related Issue

47069

Issue Date

2024-04-01

Receive Date

2023-11-29

Publish Date

2024-04-01

Page Start

48

Page End

56

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_348854.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=348854

Order

6

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION AND PHYLOGENIC ANALYSIS OF CORONAVIRUS IN DIARRHEIC CALVES USING PARTIAL SEQUENCE N GENE FROM SULAYMANIYAH, IRAQ

Details

Type

Article

Created At

28 Dec 2024