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318829

VINCRISTINE-INDUCED NEUROTOXICITY IN RATS MEDIATED BY UPREGULATION OF INOS, IBA1, NESTIN, PARP AND CASPASE 3: AMELIORATIVE EFFECT OF ERYTHROPOIETIN AND THYMOQUINONE

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Molecular biology

Abstract

Vincristine (VCR) is an effective anticancer medication, although it has neurotoxic side effects. Erythropoietin (EPO) is the main regulator of erythropoiesis. Thymoquinone (TQ) protects brain cells from oxidative stress that causes neurodegenerative disorders such as Alzheimer's and Parkinson's. This study aims to investigate the VCR toxicity on the cerebrum as well as the possible neuroprotective effects of TQ and EPO against VCR toxicity in a rat model. An intraperitoneal injection of VCR (150 μg/kg) 3 times a week for 5 weeks caused marked histopathological changes in the brain such as neuronal degeneration with aggregations of glial cells around the degenerated neurons (satellitosis), congestion of blood vessels and severe demyelination in the white matter of the cerebrum. VCR considerably increased nestin, iBA1 and iNOS expression, while synaptophysin expression decreased. It also caused upregulation of caspase 3 and PARP expression, resulting in hemorrhage, demyelination, and neuronal degeneration. Treatment of rats with TQ or EPO either alone or in combination improved histopathological changes through down-regulation of nestin, iBA1, iNOS, caspase 3 and PARP. It was concluded that EPO and TQ ameliorate the neurotoxic effect of VCR on the cerebrum, however, a synergetic effect was evident when TQ and EPO were combined.

DOI

10.21608/avmj.2023.223481.1171

Keywords

Vincristine, Brain, erythropoietin, Thymoquinone, apoptosis, Demyelination

Authors

First Name

SAHAR

Last Name

AHMED

MiddleName

A

Affiliation

Department of Molecular Biology, Molecular Biology Researches & Studies Institute, Assiut University, Assiut - Egypt.

Email

saharabdelrahman000@gmail.com

City

-

Orcid

29609242500729

First Name

HOSSAM EL-DIN

Last Name

OMAR

MiddleName

M

Affiliation

2Department of Zoology and Entomology, Faculty of Science, Assiut University, Assiut, Egypt Department of Basic Science, School of Biotechnology, Badr University in Assiut, Egypt

Email

hossameldin.mo@gmail.com

City

-

Orcid

-

First Name

MAHMOUD

Last Name

SOLIMAN

MiddleName

-

Affiliation

Laboratory of Pathology, College of Veterinary Medicine, Chonnam National University, South Korea

Email

mahmoud.soliman@vet.au.edu.eg

City

-

Orcid

-

First Name

ABO BAKR

Last Name

ABDEL SHAKOR

MiddleName

-

Affiliation

Department of Zoology and Entomology, Faculty of Science, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

MANAL

Last Name

SAYED

MiddleName

M

Affiliation

Department of Chemistry, Animal Health Research Institute, Assiut Lab.

Email

-

City

-

Orcid

-

First Name

OMNIA

Last Name

OMAR

MiddleName

HM

Affiliation

Assiut International Center of Nanomedicine, El-Rajhy Liver Hospital, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

SARY

Last Name

ABD ELGHAFFAR

MiddleName

KH.

Affiliation

Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Assiut University, Egypt 6Department of Pathology and Clinical Pathology, School of Veterinary Medicine, Badr University in Assiut, Egypt

Email

sarynasr@aun.edu.eg

City

-

Orcid

0000-0002-5956-8798

Volume

69

Article Issue

179

Related Issue

43704

Issue Date

2023-10-01

Receive Date

2023-07-26

Publish Date

2023-10-01

Page Start

172

Page End

185

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 69_Issue 179_Pages 172-185.pdf

PDF . 1.7MB

Link

https://avmj.journals.ekb.eg/article_318829.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=318829

Order

15

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

VINCRISTINE-INDUCED NEUROTOXICITY IN RATS MEDIATED BY UPREGULATION OF INOS, IBA1, NESTIN, PARP AND CASPASE 3: AMELIORATIVE EFFECT OF ERYTHROPOIETIN AND THYMOQUINONE

Details

Type

Article

Created At

28 Dec 2024