Urine and tissues samples were collected for bacteriological examination from 50 apparently diseased sheep of different ages and sexes which were clinically suspected suffering from renal infection and from slaughtered animals. We were collected samples from many locations at Matrouh Governorate and sent for laboratory. Post mortem examination of the renal system of affected cases revealed different investigations. kidneys appeared swollen, its surface was mottled red. Some kidneys were pale and greatly enlarged. Cut section of the kidney showed areas of hemorrhage and abscess formation. The results indicated that 80% (40) sheep were positive for bacterial infection however 10 show negative results of bacterial renal infection. Bacteriological investigations revealed that the isolated organisms were: Escherichia coli (20%), Staphyolcoccus aureus (25%), Pseudomonas aeruginosa (5.0%), Streptococcus spp. (7.5%), Enterococcus faeclis (7.5%), Corynebaeterium spp. (17.5%), Klebseilla pneumoniae (2.5%). and. Listeria monocytogene (2.5%). Moreover, mixed infection found in 12.5% of the examined samples. A total of eight isolates of E coli from renal examined samples were serologically positive to O111, O104, O26, O113, O91, O103 and O126.  PCR results showed that two strains for Listeria isolates were Listeria monocytogenes and some of them have InI B gene.
Antibiogram was applied upon the isolated bacterial pathogens and found that Garamycine was the drug of choice for treatment of infected animals.