Bovine viral diarrhea (BVD) is a common viral disease that can affect both domesticated and wild animals. Despite their economic losses, there isn't much information available on BVD in Assiut Governorate. Therefore, the present study's objectives were to determine the clinical findings, risk factors associations with the infection rate, and molecular diagnosis of BVD virus (BVDV). The current study was conducted on 50 cattle that belonged to different villages in Assiut Governorate, Egypt. The clinical examination findings revealed fever, oral lesions, diarrhea, respiratory symptoms, and corneal opacity. Serum samples were collected for laboratory analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) assay had been employed for BVDV diagnosis. BVDV RNA was found in the serum of seven cattle. There was no significant difference (P<0.05) between the percentages of BVD infection and the sex, age, and breed (native and mixed breed) of molecularly tested cattle. According to the climatologic circumstances of Assiut governorate, there was a discernible variation (P<0.05) between the BVD infection rate and the cold and hot months. It is advisable to emphasize how crucial it is to implement efficient preventative and control measures throughout Egypt in order to reduce the prevalence of BVDV.