This study aimed to determine the cardiac effects of two different anesthetic protocols by measuring serum levels of Troponin 1, CK-MB, NT-proBNP and D-Dimer in healthy castrated non cardiac dogs . Thirty adult, healthy, and noncardiac male dogs that were brought to the animal hospital affiliated to the Animal Health Application and Research Center of Siirt University for castration. The animals were sedated with 2 mg/kg, intramuscular xylazine HCL and intramuscular 10 mg/kg Ketamine HCL were administered. The animals were intubated and connected to the closed-circuit anesthesia device. Following the injectable general anesthesia application, one group (G1) (n=15) was administered with 2-3% Isoflurane inhaler, the other group (G2) (n=15) was administered with 2-3% Sevoflurane inhaler to maintain anesthesia. Blood samples were collected before and 12. hours after anesthesia. Results reveled non-significant changes in serum levels of CK-MB over time. However, a significant difference was observed in CK-MB values between sevoflurane and isoflurane No significant changes in Troponin values was recorded. Significant changes in Nt-Pro BNP values over time were observed but the changes were not significant between anesthetic protocols. With the present study, we can partially say that sevoflurane is safer than isoflurane, but we believe that more studies should be done with more samples.