Background: Despite recent advances, the difficulties involved in management of bone infections continue to plague the practice of orthopedics, this study reviews the clinical results using biodegradable antibiotics impregnated calcium sulfate (AICS) beads in 24 patients who suffered from osteomyelitis. Aims of the work: evaluation the role of antibiotics impregnated calcium sulfate in eradication of infection in case of osteomyelitis. all patient prepared for surgical debridement and calcium sulfate Patient and methods impregnated with vancomycin and gentamycin beads were inserted into the bone cavity and surrounding soft tissue. Results: this study include 24 patients, 17 (70.83%) patients were males while 7 (29.17%) patients were females, The mean follow-up was 9 months (range from 6-12 months), the mean age of the patients was 33.2 ± 19.5& tibia is the most common affected bone & Staphylococcus aureus was the organism that had been reported in 45.83% of all swabs, In the final results 21(87.5%) patients were completely healed while only3 (12.5%) patients still had infection. Conclusion: antibiotics impregnated calcium sulfate is safe and effective method in treatment of osteomyelitis and the results show high rate of eradication of infection.